Interrelationship of Vitamin B12, Androgens and Cortisol in Chronic Stress and Associated Vascular Dysfunction
Rubeeya Lodhi*, Dr. Aashish Panchal
Simultaneous estimation of Sildenafil citrate and Asian ginseng powder extract in Pharmaceutical dosage form -
Effervescent Tablet
Naishi Kirtikumar*, Dr. Dilip Maheshwari
Volume 4, Issue 5
Pages no. 293-334
Development and Validation of Dissolution Method for Udenafil Tabletse
Deepika H. Khubchandani
Development and Validation of UV Spectrophotometric Method for Saroglitazar Tablets
Ekta H. Amin*, Dr.Dilip G. Maheshwari
A Review On: Awareness on Chikungunya in Tribal area: An Initiative
Bhandari Krunal, Desai Chandani, Gouri Arzoo, Desai Chirag
Development and Validation of Stability Indicating Assay Method of Haloperidol in Oral Solution
Ravidan Gadhavi, Miss. Jigisha Patel
Process Optimization and Scale-Up of Anti-Hypertensive Bi-Layer Tablet Formulation-A Quick Review
Umesh Khatri, Ramesh Pareek
© JPSBR Publications 2011-2024
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ABSTRACT:
Stress,
both
physical
and
psychological,
is
attracting
increasing
attention
among
neuro
researchers.
In
the
last
20
decades,
there
has
been
a
surge
of
interest
in
the
research
of
stress
induced
manifestations
and
this
approach
has
resulted
in
the
development
of
more
appropriate
animal
models
for
stress
associated
pathologies
and
its
therapeutic
management.
These
stress
models
are
an
easy
and
convenient
method
for
inducing
both
psychological
and
physical
stress.
To
understand
the
behavioral
changes
underlying
major
depression,
molecular
and
cellular
studies
are
required.
Dysregulation
of
the
stress
system
may
lead
to
disturbances
in
growth
and
development,
and
may
this
may
further
lead
to
the
development
of
various
other
disorders.
This
article
reviews
the
interrelation
of
Vitamin
B12,
androgens
and
cortisol
in
chronic
stress
model
and
their
neurobiology,
including
the
different
neurotransmitters
and
heart
function
affected.
There
are
various
complications
associated
with
stress
and
their
management
through
various
pharmacological
and
Non?Pharmacological
techniques.
The
use
of
vitamin
b12
in
the
treatment
of
stress
related
problems
is
in
practice
in
both
Indian
and
Western
societies,
Examination
of
the
hyper-responsiveness
of
the
Hypothalamic-Pituitary-Adrenal
axis,
consequent
elevated
serum
cortisol,
Androgens
plus
the
effects
of
this
upon
brain
structure
and
function,
provides
a
model
for
understanding
how
chronic
stress
may be a causal vector in the development of major organ dysfunction like CVS dysfunction.
KEY WORDS
: Chronic Stress, Vitamin B12, Androgens, Cortisol, CVS Dysfunction, sympathoadrenal system
ABSTRACT:
Analysis
of
pharmaceutical
product
is
very
important
as
it
concerned
with
quality
of
life.
Sildenafil
citrate
is
a
PDE-5
inhibitor.
Asian
ginseng
extract
is
Panax
Ginseng
obtained
from
herbaceous
species
(Family:
Araliaceae).
Combination
of
Sildenafil
citrate
and
Asian
ginseng
extract
are
used
in
treatment
of
erectly
dysfunction.
The
objective
is
to
develop
simple,
rapid,
precise
and
validated
RP-HPLC
method
for
simultaneous
estimation
of
Sildenafil
citrate
and
Asian
ginseng
extract
in
the
pharmaceutical
dosage
form.
Chromatographic
separation
of
Sildenafil
citrate
and
Asian
ginseng
extract
was
carried
out
on
BDS
Hypersil
C18,
250mm
×
4.6mm,
5µ
(particle
size),
Thermo
scientific
column
using
mobile
phase
Acetonitrile
:
0.02M
Phosphate
buffer
(pH
3)
(60
:
40
v/v)
&
detection
at
210
nm.
Linearity
of
Sildenafil
citrate
and
Asian
ginseng
extract
were
found
to
be
25
-
75
µg/ml
and
2.5
-
7.5
µg/ml.
The
correlation
coefficient
was
found
to
be
0.9992
and
0.9995
was
found
for
Sildenafil
citrate
and
Asian
ginseng
extract
respectively.
The
%
RSD
for
precision
was
found
to
be
less
than
2
%
and
the
%
recovery
was
found
between
97-102
%.
Developed
&
validated
RP-HPLC
method
was
found
to
be
simple,
accurate,
economical,
robust
and
reproducible.
There
was
no
interference
of
the
excipients
in
the
determination
of
drugs
from
pharmaceutical
dosage form so it can be
successfully applied for routine analysis.
Keywords:
Sildenafil citrate, Asian ginseng extract, RP-HPLC method, Validation, Effervescent Tablet.
ABSTRACT:
The
aim
of
this
work
was
to
develop
and
validate
a
dissolution
test
for
Udenafil
tablet
using
spectrophotometric
method.
The
dissolution
established
conditions
were:
900
mL
of
0.1
N
HCl
as
dissolution
medium,
using
a
paddle
apparatus
at
a
stirring
rate
of
50
rpm.
The
drug
release
was
evaluated
by
UV
spectrophotometric
method
at
292
nm
for
Udenafil.
In
this
study
%
drug
release
of
Udenafil
was
found
to
be
greater
than
90%
in
45
minutes.
The
method
was
validated
to
meet
requirements
for
a
global
regulatory
filing
which
includes
linearity, precision, accuracy.
KEYWORDS:
Udenafil, Dissolution, UV spectrophotometric method, validation.
ABSTRACT:
A
new,
simple,
precise,
accurate,
reproducible
and
economical
and
sensitive
UV
Spectrophotometric
method
has
been
developed
for
the
estimation
of
Saroglitazar
in
bulk
and
pharmaceutical
dosage
form.
The
determination
was
made
at
294
nm
for
Saroglitazar
over
the
concentration
range
of
8-24
g/ml
with
mean
recovery
of
99.91%.
The
LOD
and
LOQ
were
found
to
be
0.50µg/ml
and
1.52µg/ml
respectively.
Methanol was used as solvent. The validation of method was carried out as per ICH Guidelines.
KEY WORDS:
Saroglitazar, UV Spectrophotometric method, Validation, LIPAGLYN Marketed formulation
ABSTRACT:
A
survey
was
conducted
to
know
and
spread
awareness
in
people
on
various
aspects
of
Chikungunya,
like
causative
organism,
basic
treatments,
and
contingency
and
to
know
if
they
have
seen
or
observe
any
cases
which
are
also
helpful
in
estimation
of
this
disease
burden
in
Tribal
areas
of
Valsad
district,
Gujarat.
A
well
designed
questionnaire
and
leaflet
about
disease
was
prepared
with
the
help
of
clinical
pharmacist
and
standard
WHO
questionnaire
format.
With
an
initiative
to
door
to
door
communication
to
spread
awareness
we
received
fully
filled
100
forms
and
dispatch
leaflets
on
the
disease
spreading
community
awareness.
A
percentage
analysis
for
each
question
was
carried
out.
34%
people
were
aware
about
the
viral
disease
Chikungunya,
where
36%
were
know
how
it
is
spread
and
11%
were
aware
about
its
contingency.
36%
were
aware
how
to
prevent
the
spreading
and
2%
have
seen
cases
of
Chikungunya
sufferings.
In
this
awareness
study
61%
were
male
and
39%
were
female,
85%
were
interested
to
know
about
the
disease
but
only
4%
were
ready
to
spread
awareness
on
the
disease
in
others
as
only
11%
were
graduates
so
it
might
affect
the
involvement
in
spreading
the
awareness.
There
is
a
much
need
to
spread
awareness
in
tribal
areas
to
improve
healthcare
sector
of
the
country.
For
the
improvement
of
healthcare
through
community
awareness
every
students
of
graduation
level
in
Pharmacy
must
work
together
on
same
platform
and
utilize
their
skills
and
knowledge
for
the
lifting
up
the
community
healthcare.
Awareness
programs
on
Chikungunya
and
other
parasitic
disease
have
to
be
conducted
for
the
various
semirural
and
tribal areas.
KEYWORDS:
Chikungunya, Aedes Aegypti, Aedes Albopictus, anti-viral medicines, Awareness in Tribal area.
ABSTRACT:
The
present
research
work
aims
to
develop
a
simple,
precise,
accurate,
rapid,
reproducible
and
economical
method
for
the
estimation
of
Haloperidol
by
RP-HPLC
method.
An
absorbance
maximum
for
Haloperidol
was
found
to
be
at
254
nm
using
methanol
as
a
solvent.
The
chromatography
was
performed
on
a
Restek
Pinnacle
II
C18
(250
mm
x
4.6
mm
i.d.,
5
m
particle
size)
column
with
mobile
phase
containing
Methanol:
Tetrabutyl
ammonium
hydrogen
sulphate
(55:45
v/v).
The
flow
rate
was
1
ml/min
and
the
eluent
was
monitored
at
254
nm.
The
selected
chromatographic
conditions
were
found
effectively
to
separate
Haloperidol
at
7.707
min,
Methyl
Hydroxyl
Benzoate
(MHB)
at
5.323
min
and
Propyl
Hydroxy
Benzoate
(PHB)
at
12.492
min.
Linearity
was
found
in
the
range
of
20-200
g/ml
for
Haloperidol,
20-200
g/ml
for
MHB
and
20-200
g/ml
for
PHB.
The
obtained
correlation
coefficient
was
0.999.
The
accuracy
was
evaluated
by
recovery
study
and
recovery
result
was
obtained
between
98.8%
to
100.8%
and
the
relative
standard
deviation
below
2%
was
achieved.
The
value
obtained
for
LOD
was
0.90
g/ml,
1.18
g/
ml,
0.08
g/ml
and
LOQ
was
2.75
g/ml,
3.58
g/ml,
2.62
g/ml
for
Haloperidol,
MHB
and
PHB
simultaneously.
The
proposed
method
was
found
to
be
fast,
accurate,
precise,
simple,
sensitive
and
reproducible
for
analysis
of
Haloperidol
in
Oral
Solution.
Haloperidol
was
also
subjected
to
stress
conditions
of
acid
hydrolysis,
base
hydrolysis,
oxidation,
photolysis
and
thermal
degradation
under
same
chromatographic
condition.
From
all
stability
results,
it
is
concluded
that
stability
study
of
Haloperidol
can be apply to oral solution sample of haloperidol.
Keywords:
Haloperidol, MHB, PHB, RP-HPLC, Validation.
ABSTRACT:
In
a
pharmaceutical
industry,
preparation
of
a
dosage
form
need
process
scale-
up
in
several
stages.
Pharmaceutical
scale-up
involves
manufacturing
drug
product
with
increasing
batch
sizes
on
larger
equipment.
Production
scale
is
typically
differ-ent
from
R&D
scale
in
terms
of
batch
size.
The
processing
conditions
during
R&D
scale
and
commercialize
scale
are
quite
different
from
each
other.
The
successful
established
conditions
for
a
product
at
R&D
scale
would
not
be
same
at
production
scale.
Therefore,
each
and
every
unit
operation
in
the
production
of
tablet
formulation
at
production
scale
is
optimized
for
successful
scale-up.
Therefore,
scientific
optimization
techniques
are
used
to
provide
effective,
accurate,
and
less
time
consuming
way
to
scaling
up
the
production
profile
from
laboratory
to
commercialize
plant
so
as
to
have
higher
output
without
any
objection
on
quality
matters.
In
the
present
work,
an
attempt
has
been
made
to
implement
the
quality
by
design
(QbD)
principles
for
successful
process
scale-up.
The
design
of
experiment
(DoE)
strategy
was
applied
during
the
process
scale-up
of
any
product
to
reduce
the
risk
of
batch
failure
on
quality
attributes.
Quality
by
design
approach
using
design
of
experimentation
can
be
implemented
successfully
to
provide
fully
robust
and
reproducible
process
that
will
develop
an
assured quality product.
Keywords:
Immediate Release Tablet, Immediate Release Bilayer Tablet, Anti-Hypertensive Bi-Layer, Optimization, Scale-Up
